People who want a new or replacement bus pass from Monday 24 October will need to go to Centre4 in Wootton Road, Grimsby.
Bus passes will no longer be issued from the Warehouse in Freeman Street, Grimsby.
This includes concessionary bus passes for elderly people, adults and children with a disability and, companions of adults and children with a disability.
Changes to where bus passes are issued were made in May this year when charity Centre4 was awarded the contract to issue them on behalf of North East Lincolnshire Council.
Passes were also issued at the Warehouse but the service there will end on Friday.
Cllr Matt Patrick, portfolio holder for Finance, Resources and Inclusion at NELC, said:
“Centre4 is a great venue with lots to offer visitors including a café, sports hall, and a wide range of activities for people to get involved with.
“We are working with the charity to look at other locations in the area that could be used to issue bus passes.
“Until then, bus passes will only be issued from Centre4.”
The change comes into place from Monday 24 October. Centre4 is open Monday to Friday from 9am to 5pm.
The centre is served by the number four and number three bus. For more details visit www.centre4.org.uk.